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Reference Ranges

Reference ranges for the same methods or instruments may differ between laboratories and buying viagra online safe geographic areas for the following reasons:

  1. Operating conditions are different.
  2. Criteria for selection of healthy subjects are different.
  3. Geographic areas have different temperature, altitude, barometric pressure, humidity, and time zones.
  4. Subject preparation and sample collection may differ.

Because of these differences, reference ranges should be established locally whenever a new test is generic levitra 40 mg introduced or an existing method is changed. NCCLS recommends testing at least 120 patient samples for establishment of a statistically significant reference interval. Other experts recommend a minimum of buy viagra online 50mg 200 samples to ensure stable lower and upper reference limits.

It may be necessary to viagra best prices subdivide reference ranges into partition groups. For example, hemoglobin reference ranges should be established by gender and age while cortisol ranges should be subdivided by the time of day. Each partition group should contain 120 samples.

Preanalytical factors may also need to be controlled in order to insure that accurate reference ranges are derived. Some factors to consider are: timing of collection; food, water, and alcohol consumption in the viagra in india past 24 hours; venipuncture site; tourniquet time; and type of collection tube. Samples to be used for reference range determination should be collected and processed the same as routine laboratory work.

Two approaches, parametric and nonparametric, may be taken when analyzing reference range data. The parametric approach involves calculating the mean and standard deviation to determine the range of values that fall within the 95% confidence interval. Before this method can be used the population distribution must be examined to ensure that a Gaussian (bell shaped) distribution is present. If the population distribution is skewed, parametric statistics are not valid. The population distribution can be visualized by plotting a frequency histogram in Excel. Because this exercise is very time consuming, the nonparametric approach is preferred.

The nonparametric approach involves establishing the values falling at the buy cialis discount 2.5 and 97.5 percentiles of the population as the lower and upper reference limits. The first step is to it's great! enter the analyte values in Excel and generic viagra overnight deliver western union sort them in ascending order of magnitude. The number of buying viagra online in australia values in the data set is then multiplied by 0.025 and 0.975 to obtain the percentile ranks of the upper and lower reference limits, respectively. For example, if the data set consisted of 120 specimens, the 2.5 and 97.5 percentile ranks would be calculated by multiplying;

120 x 0.025 = 3

120 x 0.975 = 117

The lower reference limit would be the third number from the where to get levitra beginning of the sorted list and the upper reference limit would be the third number from the end. The reference range would be the central 95% of the data which falls between the 3rd and 117th values.

Outliers can have a substantial effect on the calculation of reference ranges by this method and should be removed. Outliers are data points that lie outside the bulk of the population. Although computer programs and formulas exist to identify outliers, none is www.slic.de more effective than visual examination of the data.

Verification of herbal alternative to viagra a Reference Interval

Sometimes it is too difficult to collect a large number of samples to establish a new reference range. In this situation it is permissible to determine whether the manufacturer’s stated reference range, your existing reference range, or a reference range established by another laboratory using the same instrument and reagents is applicable. A minimum of 20 samples from healthy men and tramadol free shipping women (40 total) should be tested and the results compared to the buy viagra in canada published reference range. If 95% of the results fall within the published reference ranges, it can be accepted for use. The results should be evenly spread throughout the published reference range and not clustered at one end.

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