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Leukocyte Antibody (Leukoagglutinin)

Antibodies to leukocyte antigens may develop in multiply transfused patients and in multiparous women. They may produce febrile reactions to transfused blood components containing incompatible leukocytes.

The patient's serum is incubated with leukocyte suspensions from four donors. Agglutination of any of the leukocyte suspensions by the patient's serum indicates the presence of granulocyte antibodies if the same leukocytes are not agglutinated by negative control sera, lacking leukocyte antibodies. Patients with leukoagglutinins should be transfused with leukocyte poor blood components.

Results are reported as negative or positive. The reference value is negative.

Specimen requirement is on 10 mL plain red top tube of clotted blood.

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